abs

학술발표회초록보기

Inquiry on Abstract abstract@kcsnet.or.kr

Inquiry on Payment member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

114th General Meeting of Korean Chemical Society Glycopeptide Analysis of EPGs and PGIPs by Liquid Chromatography Mass Spectrometry

Submission Date :
8 / 28 / 2014 , 15 : 24 : 50
Abstract Number :
114082810868
Presenting Type:
Poster Presentation
Presenting Area :
분석화학
Authors :
김상진, 서현경, 임재민*
창원대학교 화학과, Korea
Assigned Code :
ANAL.P-558 Assigend Code Guideline
Presenting Time :
10월 15일 (수요일) 16:00~19:00
Polygalacturonase inhibiting proteins (PGIPs) are members of the leucine rich repeat family of proteins, involved in plant defense against fungal pathogens. PGIPs exhibit a remarkable degree of specificity in terms of their ability to bind and inhibit their target molecules, the endopolygalacturonases (EPGs). This specificity has been attributed for certain EPG/PGIP combinations to differences in primary sequence, but this explanation is unable to account for the full range of binding and inhibitory activities observed. In this study, we focused on the analysis of glycopeptide of EPGs and PGIPs after incubation the glycoprotein with trypsin by liquid chromatography mass spectrometry (LC-MSn). The recent development in mass spectrometry such as multiple dissociation techniques (CID and ETD) enables detailed structure determination of the glycopeptide. The LC-MSn glycopeptides analysis provides glycosylation sites and site-specific glycan structural information.