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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of SERS based point-mutated DNA detection using metal nanodendrite structure [WISET포스터상]

등록일
2014년 8월 28일 16시 01분 24초
접수번호
1182
발표코드
ANAL.P-567 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
김샛별, 정회일*
한양대학교 화학과, Korea
A point mutation is a type of serious mutation that causes the replacement of a single base nucleotide with another nucleotide of the genetic material, DNA or RNA. Because of its similarity with normal sequence, sensitive analytical method is in great demand for the detection of a point mutation. In this study, we have investigated surface enhanced Raman scattering (SERS) based sensing scheme to fulfill the necessary analytical sensitivity. We fabricated silver-gold nanodendrite structure via a simple electrochemical synthesis used it as a SERS. For the purpose, we used toehold-mediated DNA strand displacement reaction for increasing specificity of target sequence. Initially, the hybridized pair of thiol modified probe DNA (complementary to a point mutated DNA) and indicator DNA labeled with Raman dye (shorter then probe DNA) was immobilized on the surface of Ag-Au nanodendrite, thereby resulting in the increase of Raman signal due to the positioning of the Raman dye near the surface. When a target DNA was added, it pushed the indicator DNA away, so decreasing Raman intensity as a function of the concentration. In overall, the current research demonstrate sensitive and rapid detection of point mutated DNA sequence caused by Avellino corneal dystrophy using bimetallic nanodendrite SERS substrate.

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