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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Plasmid DNA as a reference molecule for Detection of Genetically Modified Rice with SYBR Green1 Dye using Real-Time Quantitative PCR

2014년 8월 28일 16시 04분 25초
BIO.P-640 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
김태옥, 한옥수1,*
전남대학교 생명공학과, Korea
1전남대학교 분자생명공학과, Korea
Owing to the policy of GMO Labeling, method for quantitative detection of GMO have to developed and improved. Presently Real-time PCR is one of the suitable method for GMO detection and estimation of transgene copy number. The purpose of our study was to developed novel standard plasmid as a reference molecule for detection of GM rice. Two exogenous target DNA fragments overexpressing the Allen Oxide Cyclase gene and one endogenous fragment of Sucrose Phosphate Synthase gene are present in this plasmid. three primers were designed for each of the exogenous and endogenous DNA fragments with two primers for the former DNA fragment and one for later one, these were tested by qualitative PCR. The specificity of these primers were further checked by generating Melting curve analysis using Real-time quantitative PCR. Standard curve was established by both plasmid DNA and genomic DNA to compare the two types of DNA calibrators using SYBR Green 1 and comparing result showed that the plasmid DNA is suitable as reference molecule. Therefore it is concluded that plasmid DNA can be used instead of genomic DNA as reference molecule.