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학술발표회초록보기

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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 BIOCOMPATIBLE COATINGS FOR BIOANALYTICAL DEVICES

등록일
2014년 8월 28일 16시 14분 39초
접수번호
1207
발표코드
ANAL.P-568 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
이진욱, 노주형1, 조원련2,*
원광대학교 바이오나노화학, Korea
1원광대학교 바이오나노화학부, Korea
2원광대학교 생명나노화학부, Korea
Protein chips need to immobilize proteins that retain their native structures. Polymer coatings can be used to build up nanostructures on a protein-chip. This is generally done by synthesizing the polymer with a series of steps. Polymer coatings reported in this study were prefabricated in solution as small polymers and then assembled into a coating in a single, or a small number of steps. This reduces the time and complexity required for the device fabrication such as microfluidic systems.
Prefabricated polysuccinimide (PSI) coatings were developed as a new generation of biocompatible coatings for microfabricated analytical systems. These coatings were developed for gold and/or glass BioCD discs. Linear and branched PSIs were characterized with NMR, elemental analysis (EA), size exclusion chromatography (SEC), and AFM. These methods established that PSI coatings deposited on surfaces were usually 2-5 nm thick and very hydrophilic. This prevents non-specific binding of proteins from the surface physically and chemically.
Proteins were immobilized on inorganic surfaces in three ways: PSI coatings with biotin, protein G, or direct binding to IgG on immunological assays. Antibody immobilization and antigen detection were measured using AFM, ellipsometry (ELS), fluorescence microscopy, and interferometry. The nonspecific binding of sample proteins from the blocking coatings was confirmed to be very low through SEC development project.

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