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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Introduction to isotope-coded carbamidomethylation-based isotope labeling for absolute quantification of serum BNP-32 using nanoLC-ESI-MS/MS

등록일
2014년 8월 28일 16시 17분 30초
접수번호
1213
발표코드
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발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
권미향, 문명희, 강덕진1,*
연세대학교 화학과, Korea
1한국표준과학연구원(KRISS) 삶의질측정표준본부, Korea
Quantifying the amount of brain natriuretic peptide-32 (BNP-32) in human serum is significant, since BNP-32 is a crucial biomarker for diagnosis of the congestive heart failure (CHF). Nevertheless, it is still challenging in determining its quantity of the low abundant BNP-32 in serum (100 pg/mL), due to absence of a proper isotope-labeling method. To address this issue, we developed a simple and novel isotope labeling method as isotope-coded carbamidomethylation (iCCM). Carbamidomethylation (CM) with iodoacetamide (IAA) and its isotope (IAA-13C2, D2) used to block the thiol group and simultaneously lead to the mass difference of 8 Da between CM- and iCCM-labeled BNP-32. The resulting BNP-32 mixture, equally pooled, introduced to nanoLC-ESI-MS/MS to evaluate the labeling efficiency, and its relative abundance ratio for matching with the spiked mass ratio. Consequently, we found that an iCCM-based isotope labeling method is one of alternative method for quantifying targeted BNP-32 in serum with high reproducibility and labeling efficiency.

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