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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Quantitative profiling of respiratory syncytial virus-induced N-glycoproteme using iTRAQ couple with online mHFER nLC-ESI-FT orbitrap-MS/MS

등록일
2014년 8월 28일 16시 18분 38초
접수번호
1217
발표코드
ANAL.P-570 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
이선정, 강덕진1,*, 홍종기2
경희대학교 나노의약생명과학, Korea
1한국표준과학연구원(KRISS) 삶의질측정표준본부, Korea
2경희대학교 약학과, Korea
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in infants and young children. An antiviral is available for treatment of RSV, but treatment is effective only when given early in the course of infection and presently there is no approved vaccine. RSV infection process involves the interaction between virus and host cell with N-glycosylation. Therefore, quantification of N-glycoproteome in RSV-induced sera is important task for early diagnosis. In order to quantify N-glycoproteins, we used lectin-based enrichment by means of online microbore hollow fiber enzyme reactor (mHFER)-nLC-ESI-MS/MS. For the purpose of RSV specific N-glycoproteomic profiling, RSV-infected mouse sera samples (eg. 3day and 7day) and control were tryptically digested and isotopically labeled with iTRAQ reagents. The labeled peptide from each mouse sera (eg. 3day, 7day, and control) mixed equally and pooled with lectin to form lectin-N-glycopeptide complexes. Finally, the resulting complexes introduce to mHFER-nLC-ESI-MS/MS to analysis lectin-specific N-glycopeptides. In consequence, we sucessfully quantify RSV-specific N-glycopeptides and these identified proteins may be useful to develop RSV-specific drug.

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