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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Wash-free immunoassay of prostate specific antigen using SERS-based microdroplet sensor

2015년 9월 7일 15시 11분 16초
ANAL2.O-26 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 16시 : 07분
분석화학 - Oral Presentation of Young Analytical Chemists II
저자 및
한양대학교 바이오나노학과, Korea
1한양대학교 생명나노공학과, Korea
We report a conceptually new immunoassay technique using SERS-based microdroplet sensor. Our proposed method offers a fast and sensitive assay applicable to PSA cancer marker in serum. The sensor used in this study is composed of four different compartments; the generation of microdroplets for fast PSA immunoreactions, the separation between immunocomplexes and supernant solution, the isolation of magnetic immunocomplex droplets, and the collection of SERS nano tag droplets for Raman detection. This technique allows a convenient immunoassay of PSA marker without any washing process since the magnetic immunocomplexes can be easily isolated by the droplet splitting into two smaller parts after the application of magnetic bar. Raman signals of the remaining SERS nano tags in the large droplet (supernatant solution) were measured for the quantitative evaluation of PSA marker. SERS signals for 240 droplets (8 Hz) were measured and averaged for the quantitative evaluation of PSA. The LOD, determined by our SERS-based microdroplet sensor, was estimated to be below 0.1 ng/mL for PSA cancer marker, and this value is much lower than the clinical cut-off value for the diagnosis of prostate cancer. In addition, rapid analysis with a tiny volume of sample is possible since all the process can be automatically carried out in the specially designed microdroplet channel. As a result, this SERS-based assay technique is expected to be a potential clinical tool for early diagnosis of prostate cancer.