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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Lipid mass fingerprinting of microorganisms by matrix-assisted laser desorption/ionization mass spectrometry

2016년 8월 30일 13시 15분 16초
ANAL.P-69 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 14일 (금요일) 11:00~12:30
저자 및
방글, 김정아, 김영환*
한국기초과학지원연구원 생의학오믹스연구팀, Korea
The rapid identification of bacteria is of increasing interest in food, biosafety, and clinical study. Due to rapid progress in the development of mass spectrometry devices and microorganism identification database during the last few years, bacteria identification by using mass spectrometry has become a very powerful and usable tool. We report a technique for rapid lipid profiling of bacteria by MALDI-TOF mass spectrometry. MALDI-TOF MS was applied to the rapid and direct detection of intact phospholipids from whole bacteria cells. The sample preparation procedure involved both depositing bacterial colonies directly onto the MALDI plate and using developed micro fluidic system. The phosphatidylethanolamines (PE) and phosphatidylglycerols (PG) in lipid mixture extracted from bacteria are observed in the MALDI-TOF mass spectra. The MALDI-TOF mass spectrum of each bacterial extract shows a distinguishing profile. In addition, the distinct discrimination of some species of gram negative bacteria was achieved by using principal components analysis (PCA) conducted on the relative peak intensities of phospholipids observed from the MALDI spectra. The experimental results demonstrate that the current technique is able of conducting rapid lipid analysis and has the potential for rapid clinical detection.