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제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Conjugated polymer and aptamer based fluorescence chemo- and bioassays

2016년 9월 9일 09시 25분 13초
POLY1-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 14시 : 25분
고분자화학 - Nucleic Acids in Materials Science – Programmable Biopolymers
저자 및
정지은, 우한영*
고려대학교 화학과, Korea
Conjugated polyelectrolytes (CPEs) are described by a π-conjugated backbone with ionic pendant groups, which make them water-soluble and/or bio-compatible. Here, we report a new potassium ion detection assay using a molecular beacon aptamer (MBA) and CPEs. The K+-specific aptamer sequence in MBA was used as a molecular recognition element. The hairpin-type MBA labeled with a fluorophore and quencher at both termini undergoes a conformational change (by complexation with CPEs) to either an open-chain form or a G-quadruplex in the absence or presence of K+ ions. Conformational changes of MBA as well as signal quenching or amplification via fluorescence resonance energy transfer (FRET) from CPEs provide clear signal trun-off and –on in the presence or absence of K+. Combination of high binding specificity of MBA and synthetic CPEs with tunable optical amplification property provided the ultimate detection sensitivity and selectivity. The successful detection of 5’-adenosine triphosphate (ATP) and tuning of detection range with a series of MBAs containing an ATP-specific aptamer sequence was also demonstrated using the same sensor scheme. In addition, a series of inhibitors were incorporated to modulate the detection range of MBA-based assay.