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제122회 대한화학회 학술발표회, 총회 및 기기전시회 Analysis of polyglycerophospholipids using isotope-labeled methylation

2018년 8월 17일 09시 55분 00초
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금 09시 : 50분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
JongCheol Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Polyglycerophopholipids (PGPLs) are divided into phospholipids (PLs) containing two gylcerols including phosphatidylglycerol (PG), bis(monoacylglycero)phosphate (BMP), hemi BMP, and bis(diacylglycero)phosphate (BDP), and PLs with three glycerols such as cardiolipin (CL), monolysocardiolipin (MLCL), and dilysocardiolipin (DLCL). PGPLs are synthesized from PG and closely related to each other. Since PGPLs have been reported to be associated with neurodegenerative diseases, accurate determination of these lipids is important. Isotope-labeled methylation (ILM) method using (trimethylsilyl)diazomethane (TMSD) can be utilized for relative quantitation of lipids without the addition of internal standard lipids. In addition, many lipids of trace amount could be detected by ILM method with low detection limit. In this study, analysis of PGPLs with ILM method was carried out using nUHPLC-ESI-MS/MS. The collision-induced dissociation (CID) experiments was conducted for the methylated standard PGPLs. The efficiency of methylation for these lipids was found to be > 97% and the degree of methylation was dependent on the number of phosphate groups (1: PG, BMP, Hemi BMP and BDP, 2: CL, MLCL and DLCL). PG and BMP, which are geometrical isomers, and three isomers of BMP can be resolved from each other. A good linear relationship between experimental mixing ratio and calculated peak area of H-/D- methylated lipids were observed. This method was applied to the investigation of PGPLs from a neuronal cell line (SH-SY5Y) which was treated with some drugs, commonly used for the Parkinson’s disease model, were quantitatively analyzed.