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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 Fabrication of PEGylated Au@Ag core-shell nanoparticles for SERS imaging of multiple biomarkers expressed on cancer cells

2019년 2월 14일 16시 43분 53초
ANAL2.O-21 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 20분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Namhyun Choi, Jaejeung Dang1, Jaebum Choo1,*
Department of Bionano Technology, Hanyang University, Korea
1Department of Chemistry, Chung-Ang University, Korea
Implementation of the use of metal nanoparticles in cellular imaging has been recently garnered much attention. We recently developed a surface-enhanced Raman scattering (SERS)-based cellular imaging technique to detect cancer phenotype markers expressed on cell surfaces. Therein, hollow gold nanospheres (HGNs) have been used as SERS nano tags since HGNs strongly enhance the Raman signal intensity of individual nanoparticles by localized surface plasmon effects through the pin holes in the particle structures. However, this technique has some limitations including desorption of Raman reporter molecules and aggregation of HGNs in aqueous condition. To resolve these problems, polyethylene glycol (PEG)-ylated silver shell-gold hollow core nanoparticles (AgHGNs) have been fabricated and used as SERS nanotags in this work. By the silver shell encapsulation, the desorption problem of Raman reporter molecules could be solved. In addition, the SERS intensity of AgHGNs was greatly enhanced over HGNs. Then these particles were encapsulated with PEGs to make them stable in different pH, temperature and salt conditions. Finally, these PEGylated AgHGNs were used for the SERS imaging of three different tumor-associated biomarkers (CD44, EpCAM and ErbB2) expressed on breast cancer cells to confirm their multiple marker detection capability.