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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 Highly sensitive molecular diagnostics using SERS-PCR for Bacillus anthracis detection

2019년 2월 14일 16시 44분 31초
ANAL2.O-20 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 16분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Yixuan Wu, Younju Joung1, Sohyun Park1, Jaebum Choo1,*
Department of Chemistry, Chung-Ang University, China
1Department of Chemistry, Chung-Ang University, Korea
The real-time polymerase chain reaction (QPCR) technique has been extensively used for the diagnosis of a wide range of infectious diseases. This technique found valuable clinical applications in the diagnosis of viral diseases but it suffers from several problems including time-consuming process and high fluorescent background noise. To resolve these problems, we developed a conceptually new surface-enhanced Raman scattering (SERS)-based PCR technique for its clinical applications. Due to its highly sensitive detection capability, it was possible to determine the concentration of target DNA in a short time by decreasing the number of PCR cycles. Consequently, SERS-PCR provides a high sensitivity and a short detection time over the conventional fluorescence-based QPCR. In the present study, we employed TaqMan-PCR to amplify a target gene, and then used the SERS detection technique for its highly sensitive detection. The combination of PCR with SERS proves to be effective for the rapid and sensitive detection of a low concentration of a target gene in a short time.