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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 Identification multidisciplinary function and analysis active chemical compound structure of natural biological resources that were collected from Southern-east asian countries

2019년 8월 21일 15시 26분 08초
ANAL2.O-7 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 18분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Yeseul Park, TaeYeong Park1, Dong-Ku Kang2,*
Chemistry, Incheon National University, Korea
1Department of Cosmetic Science & Management, Incheon National University, Korea
2Department of Chemistry, Incheon National University, Korea
This study aims to derive multi-function material from biological natural resources and also to identify its chemical structure. Candidate materials were collected from southern-east asian countries and one-hundred samples were provided for the screening of functional materials. First, dried samples were homogenized and extracted with 70% ethanol and then antimicrobial activity was identified using conventional ‘paper disc assay’ against infectious pathogens including S. pseudopneumoniae, S. aureus, E. coli, P. mirabilis, and C. albicans. Antioxidant activity was also validated by conventional ABTS(2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) assay and effect of cellular ROS concentration was characterized using 'DCF-DA'-based cell staining approach. After 1st screening of antimicrobial activity with paper disc assay, IC50 values of derived candidates were characterized for the quantification of antimicrobial activity using alamarBlue-based cell viability assay. Finally, one of the candidates was characterized which has both antimicrobial and antioxidant activities. The IC50 values of X were identified as 0.294, 2.340, 2.175 and 3.699 mg/ml against S. pseudopneumoniae, S. aureus, P. mirabilis and C. albicans respectively. Moreover, the IC50 value of antioxidant for X was calculated as 4.157 μg/ml. Then, active chemical compound was purified by fractionation using prep-HPLC. The activities of antimicrobial and antioxidant were confirmed with paper disc-based susceptibility test and ABTS assay respectively. Finally, their cellular action mechanism and biocompatibility were also identified.