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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 Performance evaluation of SERS-PCR sensors for future use in rapid and sensitive genetic assays

2019년 8월 22일 16시 09분 38초
ANAL2.O-12 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 33분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Yixuan Wu, Namhyun Choi1, Hajun Dang, Jaebum Choo*
Department of Chemistry, Chung-Ang University, Korea
1Department of Bionano Technology, Hanyang University, Korea
Herein, we report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a gene expression level but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform, and evaluated its performance by sequentially measuring the Raman signals of Bacillus anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limit of detections (LODs) than RT-qPCR under the small cycle number of 20. In particular, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15 but SERS-PCR enabled DNA detection after only 5 cycles with a LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1–1,000 pM) is wider than that for RT-qPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future. KEY WORDS: SERS-PCR assay, low PCR cycle, Bacillus anthracis,