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제124회 대한화학회 학술발표회, 총회 및 기기전시회 Proteomic analysis of human bronchoalveolar lavage fluid towards the biomarker discovery for lung cancers

2019년 9월 18일 18시 05분 23초
ANAL3-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 15시 : 10분
Analytical Chemistry - Recent Trends in Mass Spectrometry
저자 및
Min-Sik Kim
Department of New Biology, DIGST, Korea
Lung cancer is one of the most common cancers among many others and the leading cause of cancer-related mortality worldwide. Since the early diagnosis of the lung cancer is one of the main purposes in the field of lung cancer research, the sensitive detection of biomarker proteins from bronchoalveolar lavage fluid (BALF) has been suggested as a promising method to diagnose the lung cancer. BALF is a type of fluids that can be easily obtained from patients by endoscopically introduced device and it is believed that the process of collecting the fluid is relatively minimally invasive. Here, we aimed to develop a novel and comprehensive mass spectrometry-based method for in-depth single proteomic analysis of BALF by utilizing antibody-based depletion of relatively high abundant proteins from BALF. A total of 4,615 protein groups mapped to 4,535 gene names were identified by using LC-MS/MS from the pooled BALF sample. Our method was found to outperform conventional proteomic methods that analyzed BALF samples. Further study of multiple BALF samples from different patients to discover a list of potential biomarkers for lung cancer. I believe that this method developed in this study should expedite the lung cancer biomarker discovery.