abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 03월 08일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제127회 대한화학회 학술발표회 및 총회 High-speed tracking of synaptic protein interactions

등록일
2021년 2월 17일 16시 58분 42초
접수번호
0520
발표코드
PHYS1-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 10시 : 40분
발표형식
심포지엄
발표분야
Physical Chemistry - Recent Research Trends in Biophysical Chemistry
저자 및
공동저자
Min Ju Shon
Department of Physics, POSTECH, Korea

Molecular observations of synaptic proteins provide a unique viewpoint on neurotransmission that complements electrophysiology measurements. In this talk, I will introduce molecular tweezers that can monitor the zippering of a single SNARE complex, a process that drives synaptic vesicle fusion. Using a magnetic particle, the method simultaneously manipulates and probes the fine structural dynamics of a biomolecular assembly with a nanometer-millisecond spatiotemporal resolution. Application of the method revealed partially zippered SNAREs and their interactions with other presynaptic proteins. We found that the distinct intermediate forms of a SNARE complex are differentially regulated by associated factors such as complexin and synaptotagmin. Importantly, the addition of calcium ions to the synaptotagmin-1−SNARE complex strongly drives the complete zippering of SNARE proteins, suggesting the critical role for synaptotagmin in synchronous, Ca2+-triggered neurotransmission. I will also discuss how such a force application technique can be employed to address mechanical aspects of molecular neuroscience.


상단으로