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제127회 대한화학회 학술발표회 및 총회 An NIR dual-emitting/absorbing inorganic compact pair: a self-calibrating LRET system for homogeneous virus detection

등록일
2021년 3월 5일 18시 45분 57초
접수번호
1687
발표코드
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발표시간
목 13시 : 40분
발표형식
심포지엄
발표분야
Analytical Chemistry - Convergence Analytical Chemistry for Disease Diagnosis
저자 및
공동저자
Joonseok Lee
Molecular Recognition Research Center, Korea Institute of Science and Technology, Korea
Many conventional optical biosensing systems use a single responsive signal in the visible light region, limiting their practical applications as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair comprised NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as a donor and NIR absorbing LnNPs as an acceptor, which had a narrow absorption peak (800 nm) and showed long-term stability, enabling stable LRET with built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in HEPES buffer and 56 clinical samples. Hence, inorganic LnNP pairs may prove effective in self-calibrating LRET systems in the background-free NIR region.

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