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128th General Meeting of Korean Chemical Society Efficacy of Natural Antibacterial Feed in Paralichthys Olivaceus using Proteomics

Submission Date :
8 / 26 / 2021 , 16 : 10 : 11
Abstract Number :
Presenting Type:
Oral Presentation
Presenting Area :
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
Authors :
Junghoon Kang, Youngjin Kim, Wonryeon Cho*
Department of Chemistry, Wonkwang University, Korea
Assigned Code :
ANAL2.O-6 Assigend Code Guideline
Presenting Time :
FRI, 09 : 30
Antibiotics and chemotherapeutics have been frequently used to treat bacterial infection in order to prevent mass mortality in aquaculture products such as Paralichthys olivaceus (P. olivaceus). However, these compounds may eliminate beneficial bacteria and produce antibiotic-resistant pathogenic strains, and thus remain as residues in the human body. This study aims to analyze the efficacy of antibacterial feed by detecting proteome alteration of P. olivaceus after feeding antibacterial feed, which is being developed using natural amino acids generated from animals and plants. One group of P. olivaceus was fed antibacterial feed while another group was fed general feed for four weeks, and then both groups were artificially infected with Streptococcus parauberis. Proteins were extracted with RIPA buffer from homogenized head kidney tissues of both P. olivaceus groups. Then each supernatant fluid was trypsin digested and desalted respectively. After that, the proteins in both groups were identified with nLC-MS/MS and protein database searches. Proteins were significantly identified only in the group fed with antibacterial feed, and the efficacy of the antibacterial feed was analyzed by performing protein functional analysis.