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128th General Meeting of Korean Chemical Society Colorimetric Detection for Receptor Binding Domain of SARS-CoV-2 virus for Developing Paper-based Immunoassay

Submission Date :
8 / 31 / 2021 , 03 : 10 : 42
Abstract Number :
128083146357
Presenting Type:
Oral Presentation
Presenting Area :
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
Authors :
Xiaoyue Xu, In Geol Choi1,*
Department of Biotechnology, Korea University, China
1Department of Biotechnology, Korea University, Korea
Assigned Code :
ANAL2.O-15 Assigend Code Guideline
Presenting Time :
FRI, 10 : 24
Abstract Since the COVID-19 caused an outbreak in December 2019, millions of people in the world have been infected with the virus because of its high speed of transmission and the number of infections is still increasing, regardless of vaccination. The most common diagnostic method used in clinics is RT-PCR because it is the most accurate method but time-taking. Fast, simple, accurate and self-available diagnostic methods have been explored constantly to avoid infection. To develop a Do-It-Yourself (DIY)) diagnostic kit, paper-based colorimetric immunoassay was considered. This method is based on the interaction between antigen (RBD of SARS-CoV-2) and antibody (H11-D4). We employed Aryl Acylamidase (AAA) as a reporter that gives a color change. We cloned and overexpressed an antibody protein fused with AAA (H11-D4-AAA) to bind the spike protein of SARS-CoV-2. The binding affinity of H11-D4-AAA against RBD was determined as 131nM using surface plasmon resonance and the detectability also confirmed by dot blot assay. The detection limit of this colorimetric method was lower sensitivity than RT-PCR when the image analysis was applied (e.g. photographing using smartphone). Keywords: DIY, paper-based colorimetric immunoassay, SARS-CoV-2, aryl acylamidase Acknowledgment This work was supported by Center for Women In Science, Engineering and Technology(WISET) grant funded by the Ministry of Science and ICT(MSIT) under the team research program for female engineering students.