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128th General Meeting of Korean Chemical Society Lipidomic profiling in serum and liver tissue of mice with nonalcoholic steatohepatitis (NASH)

Submission Date :
8 / 31 / 2021 , 15 : 16 : 43
Abstract Number :
Presenting Type:
Oral Presentation
Presenting Area :
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
Authors :
Jiaxin Geng, Youngae Jung1, Geum-Sook Hwang1,*
Western Seoul Center, Korea Basic Science Institute, China
1Western Seoul Center, Korea Basic Science Institute, Korea
Assigned Code :
ANAL2.O-8 Assigend Code Guideline
Presenting Time :
FRI, 09 : 42
Nonalcoholic fatty liver disease (NAFLD) has become a global health problem, affecting 25% adult population. NAFLD is a spectrum disease ranging from simple steatosis, to nonalcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC). However, there is still not enough data for lipid alteration in the process of NASH. In this study, we examined the lipidomic changes in serum and liver tissue of mice (n=16) using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/QTOF-MS). Control and NASH groups were fed with chow diet (n=8) and NASH diet (n=8) for 3 weeks, respectively. Score plots derived from principal component analysis (PCA) showed separations between control and NASH groups. Total 14 classes of lipids were identified from LC-MS data and then Mann-Whitney test was used to show the alterations of lipids in serum and liver tissue. Free fatty acids (FFAs) such as poly unsaturated FAs (PUFAs) showed similar decreases in both serum and liver tissue in NASH group. On the other hands, phospholipids and glycerolipids differed in NASH group between serum and liver tissue. This study demonstrates that UPLC-MS/MS based lipidomic profiling is a useful tool in detecting characteristic changes at early stages of NASH in serum and liver tissue.