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129th General Meeting of Korean Chemical Society & Exposition Crosslink of metabolic alternations between liver and adipose tissues in mouse models with nonalcoholic steatohepatitis (NASH)

Submission Date :
2 / 17 / 2022 , 16 : 17 : 27
Abstract Number :
129021730347
Presenting Type:
Oral Presentation
Presenting Area :
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
Authors :
Jiaxin Geng, Youngae Jung1,*, Geum-Sook Hwang2,*
Western Seoul Center, Korea Basic Science Institute, China
1Western Seoul Center, Korea Basic Science Institute, Korea
2Korea Basic Science Institute, Korea
Assigned Code :
ANAL2.O-14 Assigend Code Guideline
Presenting Time :
FRI, 09 : 52
Nonalcoholic fatty liver disease (NAFLD) is recently recognized as one of the most prevalent etiologies of chronic liver disease, affecting 25% adult population worldwide. NAFLD represents a wide spectrum of diseases ranging from nonalcoholic fatty liver (NAFL) and the progressive form as nonalcoholic steatohepatitis (NASH). However, there is a lack of investigation summarizing organ metabolic changes in NASH model. In this study, we examined the metabolic changes in liver and adipose tissues of mice (n=47) using liquid chromatography/triple quadrupole mass spectrometry (LC/TQ-MS) for polar extracts. Control group was fed with chow diet for 3 weeks whereas NASH groups were fed with high fat diet (HFD) for 3,10,33 and 43 weeks, and mice were sacrificed at each time point. Total 8 classes of polar metabolites were found from semi-targeted polar analysis and then Mann-Whitney test was performed to compare alternations between group and ahead group along NASH progression in liver and adipose tissues. For polar metabolites, amino acids represented similar trend along the period of NASH diet fed in liver and adipose tissues. Intriguingly, change of branched chain amino acids and aromatic amino acids first appeared in liver tissues compared with in adipose tissues. This study demonstrates LC-MS/MS based metabolic profiling is a useful tool to detect characteristic changes along HFD feeding period in liver and adipose tissues.