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129th General Meeting of Korean Chemical Society & Exposition Metabolomic analysis of brain tissues from mice exposed to ambient particulate matter using LC-MS approach

Submission Date :
2 / 17 / 2022 , 16 : 20 : 11
Abstract Number :
129021730357
Presenting Type:
Oral Presentation
Presenting Area :
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
Authors :
Seo Young Jang, Geum-Sook Hwang*
Western Seoul Center, Korea Basic Science Institute, Korea
Assigned Code :
ANAL2.O-15 Assigend Code Guideline
Presenting Time :
FRI, 09 : 56
Ambient particulate matter(PM) has become major environmental risk factors to public health, and related to human diseases including respiratory disease, cardiovascular and nervous disorder. However, the effect of exposure to PM has not been fully understood in a biological system. We observe the metabolic changes in response to PM with a diameter 2.5-10 µm (PM10) exposure in mouse model. In this study, C57BI/6J mice (male) were exposed to PM10 or clean air for 3 weeks in chambers. We conducted a targeted analysis of cortex and cerebellum from mice to assess the metabolic perturbations using ultra performance liquid chromatography/triple-quadrupole mass spectrometry (UPLC/TQ-MS). Partial least squares - discriminant analysis (PLS-DA) score plots showed a clear separation between the PM10 exposed group and the control group. We observed the changes in endogenous metabolites related to neurotransmitter and amino acids including alanine, aspartate and glutamate metabolism and valine, leucine and isoleucine biosynthesis in PM10 exposed mice compared to control. Branched-chain amino acids (BCAA) were significantly upregulated in PM10 exposed group, indicating the alteration in BCAA metabolism. This study demonstrates that the LC-MS based-metabolic profiling can be used to understand the metabolism of PM10 exposed group on biological systems.