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130th General Meeting of Korean Chemical Society Evaluation of injectable hydrogel for retinal epithelial cell regeneration by varying molecular weight of poly vinyl alcohol.

Submission Date :
8 / 4 / 2022 , 10 : 40 : 20
Abstract Number :
Presenting Type:
Poster Presentation
Presenting Area :
Industrial Chemistry
Authors :
Seung Ho Choe, Se Eun Kim, Youngjun Son, Taewoong Kang, Jeong Eun Song, Gilson Khang1,*
Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Korea
1PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Korea
Assigned Code :
Presenting Time :
Retinal pigment epithelium (RPE) does not self-renew, and when it degenerates, visual dysfunction or blindness can occur. Therefore, RPE cell transplantation is one of the promising treatments. Hydrogels are widely used in tissue engineering as cell and drug carriers by mimicking the extracellular matrix. In this experiment, polyvinyl alcohol (PVA) and gellan gum (GG) were used to prepare a hydrogel suitable for delivery of RPE cells. PVA has excellent mechanical properties, biocompatibility, and high content, so it is widely used as a substitute for vitreous bodies. The high hydrophilicity of PVA prevented cells from adhering to hydrogel, so GG was mixed to increase cell adhesion. This study aimed to confirm the molecular weight of PVA suitable as an RPE cell carrier. To confirm the compatibility of RPE cells, cytotoxicity was evaluated. In addition, RT-PCR analysis was performed to confirm the expression of a specific gene in RPE cells. As a result, the cytotoxicity was low, and the higher the molecular weight, the better the cell adhesion and proliferation. The results show that the PVA/GG hydrogel has the potential to be used as a carrier for RPE cells. This work was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning (2020R1A2C2103089)