Inquiry on Abstract abstract@kcsnet.or.kr

Inquiry on Payment member@kcsnet.or.kr

130th General Meeting of Korean Chemical Society Fabrication of acetylated polyrotaxanes immobilized surface with temperature-responsive properties for cell sheet engineering

Submission Date :
8 / 4 / 2022 , 11 : 33 : 07
Abstract Number :
Presenting Type:
Poster Presentation
Presenting Area :
Industrial Chemistry
Authors :
Taewoong Kang, Atsushi Tamura1, Yoshinori Arisaka1, Hiroki Masuda2, Asato Tonegawa1, Nobuhiko Yui1, Gilson Khang3,*
Bionanotechnology and Bio-Nano Convergence Engineering, Jeonbuk National University, Korea
1Department of Organic Biomaterials, Tokyo Medical and Dental University, Japan
2Department of Maxillofacial Surgery, Tokyo Medical and Dental University, Japan
3PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Korea
※ You will get a confirmation e-mail on approval or disapproval of the author's
overseas affiliation in a few days.
Approved : 5 cases
Assigned Code :
Presenting Time :

Scaffold-free cell sheet engineering is a promising technique used in tissue engineering to regenerate damaged tissue such as cornea, heart, liver, etc. Typically, cell sheets can be harvested noninvasively by thermo-sensitive poly(N-isopropylacrylamine) immobilized culture dishes when temperature decrease because the surface changes from hydrophobic to hydrophilic. The detached cell sheets form cell-cell junctions and ECM and contain various growth factors. Polyrotaxanes (PRXs) have attracted considerable attention because of their dynamic properties resulting from the molecular mobility of the threaded cyclic molecules along with the axle polymer. Previously, we reported on acetylated PRXs (Ac-PRXs), which are composed of 36% acetylated α-CDs, poly(ethylene glycol) (PEG; Mn=10,000), and adamantyl groups, and their solubility and temperature-responsive properties in aqueous solutions were investigated. Herein, we designed novel Ac-PRX with temperature-responsive properties for cell sheet engineering. It is consisted by 36% acetylated α-CDs and PEG-b-poly(benzyl methacrylate) triblock copolymer. The Ac-PRX immobilized surfaces were successfully prepared. We confirmed that NIH/3T3 cells adhered and proliferated on the Ac-PRX immobilized surface at 37 °C, but the cell sheets were separated from the surface at 10 °C. Therefore, Ac-PRX immobilized surfaces with temperature-responsive properties are considered a new type of material for cell sheet engineering. This work was supported by the material, parts technology development project (package type) (20013651, Development of an aseptic sterilizer for producing biopharmaceuticals by control pressure 0.01Mpa 1.0 precision based on AL Fuzzy) funded by the Ministry of Trade, industry & Energy (MOTIE, Korea).