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130th General Meeting of Korean Chemical Society Evaluation of gellan gum/methacrylated gellan gum hydrogel with enhanced mechanical strength using LAP for transporting corneal endothelial cells

Submission Date :
8 / 5 / 2022 , 13 : 43 : 39
Abstract Number :
Presenting Type:
Poster Presentation
Presenting Area :
Industrial Chemistry
Authors :
Jinsol Seo, Se Eun Kim, Seung Ho Choe, Taewoong Kang, Jeong Eun Song, Gilson Khang1,*
Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Korea
1PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Korea
Assigned Code :
Presenting Time :
The lack of implantable corneas and immune response after corneal transplant surgery are big issues around the world. Gellan gum (GG) is a natural polymer with excellent biocompatibility, and it is easy to bind with other molecules due to its dynamic functional group, so that it has flexibility to chemical modification. In this study, GM hydrogel mixed with methacrylate gellan gum (MAGG) and GG was fabricated to improve the mechanical properties of the existing GG hydrogel, followed by photo-crosslinking with LAP as a photo-initiator, and it was named GM/LAP hydrogel. GM and GM/LAP hydrogels were characterized for their physicochemical and mechanical properties, and transparency to confirm their suitability for transporting corneal endothelial cells (CEnCs). In addition, in vitro study was performed with cell viability and proliferation tests, morphology analysis, and gene expression evaluation. The mechanical properties of the GM/LAP hydrogel were improved compared to the GM hydrogel. Also, CEnC cultured on GM/LAP hydrogel showed enhanced viability, proliferation and corneal-specific gene expression than CEnC cultured on GM hydrogel and cell-secreted ECM was observed. GM/LAP hydrogel with improved crosslinking can be applied as a potential cell carrier in corneal tissue engineering. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(2020R1I1A1A01073657)