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Label Free Quantitative Detection of DNA Hybridization at Glass/Air Interface using Brewster Angle Microscopy (BAM)

Submission Date :
2 / 12 / 2008 , 11 : 03 : 21
Abstract Number :
Presenting Type:
Poster Presentation
Presenting Area :
Authors :
박진희, 한종훈
포항공과대학교 화학과,
Assigned Code :
30P193포 Assigend Code Guideline
Presenting Time :
목 <발표Ⅰ>
We developed a sensitive non-labeling technique for DNA hybridization detection at glass/air interface using BAM. The probe DNA was microarrayed by microspotter onto the glass surface, which was pre-modified with epoxy functional groups. The hybridization was performed with complementary target probes. The discrimination of the hybridized spots from unhybridized ones was made using BAM. We could discriminate the lowest target concentration in the range femto molar and get quantitative results. Conclusively, this method is low cost and high sensitive, and can be applied to biological sensing techniques including immunoassay over DNA microchips.