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  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Effect of mismatches of cis-syn cyclobutane pyrimidine dimer on the thermal and base pair stabilities

등록일
2008년 2월 12일 17시 13분 12초
접수번호
1031
발표코드
31P42포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
강영민, 이준화
경상대학교 화학과,
The cis-syn cyclobutane pyrimidine dimer (CPD) is one the major classes of cytotoxic, mutagenic and carcinogenic DNA photoproducts induced by ultraviolet (UV) irradiation. XPC-hHR23B binds preferentially to bubble structure and bulky DNA but poorly recognizes CPD damaged DNA. In order to understand the molecular mechanism of the XPC-hHR23B binding to CPD lesion, we performed the thermodynamics studies on the CPD damaged DNA duplexes containing the matched T∙A base pair (CPD/AA) or mismatched T∙G base pairs (CPD/GA or CPD/GG) or T∙A base pair (CPD/TA) at the lesion site. Our study clearly explains why XPC-hHR23B easily recognizes the double T∙G mismatched CPD lesion but rarely does the matched or single T∙G mismatched CPD lesions. The single T∙G mismatch of CPD lesion maintain normal B-form helix but its double T∙G mismatches caused severe helical distortion. In addition, the double T∙G mismatches of CPD lesion induced the remarkable helical instability that might contribute to the CPD recognition by XPC-hHR23B.

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