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  • 02월 22일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Development of a Kinetic Method for the Determination of enantiomeric compositions of Biomolecules by Tandem Mass Spectrometry

등록일
2008년 2월 12일 17시 16분 47초
접수번호
1035
발표코드
금16B3심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 30분
발표형식
심포지엄
발표분야
분석화학 - New Technology for Chemical Analysis
저자 및
공동저자
이용일
창원대학교 화학과,
A fixed ligand (FL) version of the kinetic method was applied to rapid, simple, and accurate chiral analysis of biomolecules. Singly charged clusters containing the transition metal ion CuII, pyridyl ligands which serve as a fixed ligand, some amino acid as a reference, and the analyte DOPA were generated by electrospray ionization. The chiral selectivity (Rchiral), the ratio of the two fragment ion abundances when the cluster contains one pure enantiomer of the analyte expressed relative to that for the other enantiomer, varies with fixed ligands, references, and transition metals. Chiral discrimination was optimized in 1,10-phenanthroline as a FL, L-Phe and L-Pro as a reference, and Cu(II) as a central metal ion. Quantitative determinations of the enantiomeric composition of DOPA and thyroxines were achieved using two-point calibration curves. The linear relationship between the logarithm of the fragment ion abundance ratio (ln R)and enantiomeric compositions (ee%) of the molecules allows the determination of the chiral purity of enantiomeric mixtures.

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