abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Electrochemical Detection of DNA hybridization using DNA cleavage

등록일
2008년 2월 13일 14시 57분 06초
접수번호
1123
발표코드
35P240포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
전기화학
저자 및
공동저자
권도형, 김규원1, 곽주현
한국과학기술원 화학과,
1인천대학교 화학과,
We report the new method for detection of DNA hybridization using enzymatic cleavage. The strategy is based on that S1 nuclease is able to specifically cleave only single strand DNA, but not double strand DNA. The capture probe DNA, thiolated single strand DNA labeled with electroactive ferrocene group, was immobilized on a gold electrode. After hybridization of target DNA of complementary and noncomplementary sequences, nonhybridized single strand DNA was cleaved using S1 nuclease. The difference of enzymatic cleavage on the modified gold electrode was characterized by cyclic voltammetry, differential pulse voltammetry. We successfully applied this method to the sequence-selective discrimination between perfectly matched and mismatched target DNA including a single-base mismatched target DNA.

상단으로