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  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Expression and purification of dominant negative protein kinase C isoforms using MBP fusion expression system

등록일
2008년 2월 13일 17시 16분 26초
접수번호
1149
발표코드
31P45포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김초롱, Julhash U. Kazi, 소재원
인하대학교 화학과,
Protein kinase C (PKC) isoforms are a family of serine/threonine kinases divided into three subfamilies: classical, novel & atypical, and are important modifiers of several cardiovascular phenomena, carcinogenesis & apoptosis and also promising therapeutic target for cancer and other indications. In our present study, we expressed and purified dominant negative mutants of PKC isoforms. We used pMBPN3 vector to construct expression vectors. The expression vectors were transformed into BL21(DE3) E.coli strain and expressed by IPTG induction. Proteins were expressed as MBP fusion protein and purified by amylase resin. Expression levels were checked by SDS-PAGE analysis. We will use these proteins to check interaction with various PKC interacting proteins to identify whether kinase activity is required for interaction or not.

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