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  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Expression and Purification of goldfish heat-shock protein 70 (HSP70) in Escherichia coli

등록일
2008년 2월 14일 09시 58분 00초
접수번호
1239
발표코드
31P53포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김소선, 이윤주, 여명, 박장수
부산대학교 화학과,
Heat shock proteins (HSPs), also called stress proteins, are a group of proteins that are present in all life forms. Heat-shock protein 70 (HSP70) can be induced when a cell undergoes various types of environmental stresses like heat, cold and toxic compounds. HSP70 was used as a biomarker of toxicant exposure in vitro. Goldfish HSP70 was selected in our study as a biomarker for evaluating aquatic toxicity of chemicals. Escherichia coli (E. coli) have advantages with regard to ease of use and high yield of protein. Bacterial cells expressing goldfish HSP70 have been developed as new tools to predict the toxicity of chemicals. The successful expression and purification of goldfish HSP70 should increase the availability of biomarker and the generation of antibodies for further biochemical and other biological studies.

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