abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Expression, Purification and NMR Structural Studies of a Transmembrane Peptide, Syndecan-4

등록일
2008년 2월 14일 12시 39분 47초
접수번호
1363
발표코드
31P68포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
박태준, 이민혜, 김용애
한국외국어대학교 화학과,
Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family and they are present on most cell types. Syndecan-4 is known to bind to various molecules, such as basic fibroblast growth factor, midkine, and tissue factor pathway, via its heparan sulfate chain, and is thought to play many biological roles through its binding bio-mediators. To get better understand the mechanism and function of Syndecan-4, it is critical to elucidate the three-dimensional structure of it. Unfortunately, Syndecan-4 is a membrane-bound protein that transverse the cell membrane, so large-scale production of such integral membrane proteins has been limited due to insufficient yields and low solubility of protein. And also it is hard to characterize the membrane-bound three-dimensional structure using conventional solution NMR and X-ray crystallography. In this study, we demonstrate the successful high level expression and purification of Syndecan-4 transmembrane domain to facilitate structural studies in complex with membrane environments by solid-state NMR spectroscopy.

상단으로