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  • 02월 22일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Design and analysis of surface capture DNA microarray platforms

등록일
2008년 2월 15일 15시 56분 30초
접수번호
1422
발표코드
목8J3심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 15시 : 00분
발표형식
심포지엄
발표분야
고분자화학 - Bio-Related Polymers
저자 및
공동저자
David W. Grainger
Departments of Pharmaceutics and Pharmaceutical Chemistry, and Bioengineering, University of Utah, USA,
Nucleic acid microarrays represent a growing diagnostics market with billion US$ dollar potential. However, these assays have substantial performance problems to solve before they will receive clinical (FDA) approval or can be reliably produced on the nano-scale. We have used many different surface analytical methods to correlate the immobilized DNA surface state on these microarray surfaces to their performance under relevant bio-assay conditions. Immobilized DNA density, orientation, and DNA target capture capabilities on both model gold and commercial polymer diagnostic surfaces in buffer, cell lysate and serum have been compared. This is then correlated with surface analysis both before DNA immobilization to understand DNA binding chemistry, and after DNA target capture assay to understand surface capture efficiency, surface density and assay signal. All of this can be corroborated and quantified using radiolabeled DNA or label-free sensing to provide a comprehensive surface picture for how these DNA assays work and how to improve their diagnostic performance.

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