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  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Highly Photostable Core/Shell Nanoparticles Composed of a Fluorescent polymer and Silica

2011년 8월 5일 16시 22분 28초
Ⅰ-BIO.P-235 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 <발표Ⅰ>
저자 및
이창수, 정봉현1
한국생명공학연구원 바이오나노연구단, Korea
1한국생명공학연구원 바이오나노연구센터, Korea
A variety of fluorescent nanoparticles have been developed for demanding applications such as optical biosensing and fluorescence imaging in live cells. Silica-based fluorescent nanoparticles offer diverse advantages for biological applications. For example, they can be used as labeling probes due to their low toxicity, high sensitivity, resolution, and stability. In this research, a new class of highly fluorescent, efficient nanoparticles composed of a newly synthesized poly[di(2-methoxy-5-(2-ethylhexyloxy))-2,7-(9,9-dioctyl-9H-fluorene)] (PDDF) core and a silica shell (designated as PDDF@SiO2) were prepared using a simple reverse micelle method, and their fluorescent properties were evaluated using methods such as single-dot photoluminescence measurements. The enhanced photostability of the particles and their potential applications for bioanalysis are discussed in this article. The morphology, particle size distribution, and fluorescent properties of PDDF@SiO2 nanoparticles were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and photoluminescence spectroscopy. The particle size distribution, which was approximately 60 nm, resulted in excellent colloidal stability in a physiological environment. The photobleaching dynamics, total numbers of emitted photons (TNEP) and statistical measurements of individual nanoparticles were observed using laser scanning fluorescence microscopy to assess the structure and photostability of PDDF@SiO2 nanoparticles. Additionally, PDDF@SiO2 nanoparticles were used in cell toxicity and permeation tests for biological analyses, thus demonstrating the potential to use PDDF@SiO2 nanoparticles as powerful, novel materials within the emerging fields of biosensing and biomedical engineering.