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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 SPR biosensor based on immobilized E.coli cells with autodisplayed Z-domains

2012년 2월 16일 16시 41분 20초
BIO.P-692 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
이은행, 유구, 박민, 변재철
연세대학교 신소재공학과, Korea
The Z-domains of protein A was expressed as a fusion protein at the outer membrane of E.coli by using the autodisplay technology. Because of the specific affinity towards the Fc region of immunoglobulins (IgG’s), the Z-domains have been used for the orientation control of antibodies in order to improve the sensitivity of immunoassays. In this work the E.coli with autodispalyed Z-domains was immobilized as a monolayer to the SPR biosensor by the charge interaction. The surface modification was carried out by senquential layering of parylene-H film with formyl groups and poly-L-lysine, and then the E.coli cells were immobilized by the charge interaction. The effectiveness of this layer for the immobilization of E.coli was estimated by counting the number of E.coli cells in comparison with bare gold surface and poly-L-lysine coated gold surface. For the test of feasibility of the immobilized E.coli cells to SPR biosensor, the stability of immobilized E.coli cells was estimated by treatment of concentrated salt solution to the immobilized E.coli cells which were bound through the charge interaction. From this test, the E.coli cells immobilized to the parylene-H film with poly-L-lysine coating were determined to be stable under the salt concentration of human serum. Then, the applicability of the immobilized E.coli cells with autodisplayed Z-domains was demonstrated by detection of C-reactive protein (CRP). The effect of orientation control by autodisplayed Z-domains was estimated by comparing the sensivities by immobilization through the physical adsorption and charge interaction to poly-L-lysine coated layer.