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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Simultaneous determination of alkaloids in Corydalis Tuber using ultra-performance liquid chromatography and chaotropic mobile phase additives

2012년 2월 23일 15시 50분 32초
ANAL.P-639 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
김정현, 양동혁, 한상범
중앙대학교 약학대학, Korea
A new ultra-performance liquid chromatography (UPLC) method using chaotropic mobile phase additives was established to separate and determine tertiary and quaternary isoquinoline alkaloids in Corydalis Tuber. Chaotropic agents employed mostly in reversed-phase liquid chromatography are small inorganic salts (e.g. NaBF4, NaClO4 and NaPF6). Primarily with anions in the chaotrope, they can bring out beneficial effects on the chromatographic separation of basic compounds. Analytical target compounds were eight isoquinoline alkaloids, including four tertiary alkaloids (glaucine, protopine, tetrahydropalmatine and corydaline) and four quaternary alkaloids (coptisine, palmatine, berberine and dehydrocorydaline). The optimal column for UPLC method was Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) and the optimal mobile phase was consisted of 10 mM potassium dihydrogen phosphate in 30 mM NaPF6 (sodium hexafluorophosphate) and acetonitrile. The column temperature was set at 25°C and the detection wavelength was 280 nm. The developed UPLC method was successfully validated in terms of linearity, precision, accuracy, limit of quantitation (LOQ) and recovery. This UPLC method was applied to determine eight isoquinoline alkaloids in 30 Corydalis Tuber samples and multivariate analysis was performed using principal component analysis to differentiate the 30 samples. These analytical methods could be valuable for the quality control of Corydalis Tuber and its pharmaceutical preparations.