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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Chemical 'omics' Approach for Understanding Protein Cysteine Oxidation by Reactive Oxygen Species (ROS)

2012년 2월 27일 15시 52분 13초
BIO1-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 14시 : 30분
생명화학 - Reactive oxygen species in age-related diseases
저자 및
계명대학교 약학대학, Korea
Oxidative cysteine modifications have emerged as a central mechanism for dynamic post-translational regulation of all major protein classes and correlate with many disease states. Elucidating the precise roles of cysteine oxidation in physiology and pathology presents a major challenge. Recent advances in chemical biology allow for the direct detection of specific cysteine oxoforms based on their distinct chemical attributes. In addition, new chemical reporters of cysteine oxidation have enabled in situ detection of labile modifications and improved proteomic analysis of redox-regulated proteins. Progress in the field of redox proteomics should advance our knowledge of regulatory mechanisms that involve oxidation of cysteine residues and lead to a better understanding of oxidative biochemistry in health and disease. Here we report on the development of chemical and immunochemical methods for detecting sulfenic acid, the initial oxidation product that results when a thiolate reacts with H2O2. Applying these approaches to several systems, including cancer cell lines, shows it can be used to monitor differences in thiol redox status and reveals a diverse pattern of sulfenic acid modifications across different subtypes of breast tumors. These studies demonstrate the utility of these reagents for quantitatively profiling thiol oxidation associated with pathological conditions such as breast cancer and these strategies can be used as diagnostic tools for cancerous diseases.