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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Synthetic study of modified deoxynucleotides for DNA sequencing technology

2012년 2월 29일 13시 55분 36초
ORGN.P-960 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
김은선, 조용서1, 민선준
한국과학기술연구원 뇌의약연구단, Korea
1한국과학기술연구원 생체과학연구본부, Korea
DNA sequencing technology is a fundamental tool for biological research and medical diagnostics. In the early years, the majority of DNA sequencing has relied on some version of the Sanger sequencing method. However, over the past decade, many next generation sequencing (NGS) methods have been developed for dealing with massively parallel DNA sequencing in inexpensive cost. DNA sequencing by synthesis (SBS) using cleavable fluorophore functional groups as terminators is an efficient approach to compensate the limitations of current DNA sequencing techniques. Thus, it is very important to develop new fluorogenic dNTPs, which are applicable to commercial DNA SBS platforms such as Illumina. Herein, we report design and synthesis of new modified nucleotide analogues having a reversible terminator at the 3′-OH position. We are expecting that these new nucleotides derived from azidoether play a dual role as a fluorescent signal reporter as well as a protective group to block polymerase reaction for SBS. The fluorophore on the DNA extension products is able to determine a type of the incorporated base and will be easily removed under DNA compatible conditions to regenerate a free 3’-OH group to reinitiate the polymerase reaction.