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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Automated Multidimensional and Multifunctional Ultra-High-Pressure Liquid Chromatography/ Tandem Mass Spectrometry

2012년 3월 1일 15시 34분 41초
ANAL.P-645 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
이정화, 이상원
고려대학교 화학과, Korea
Automated multidimensional and multifunctional ultra-high-pressure liquid chromatography (UPLC) system/tandem mass spectrometry that allows 1-dimensional, 2-dimentional separation (strong cation exchange/reverse phase liquid chromatography), and online phosphopeptides enrichment by using a single binary nano-flow pump is developed. With the switching of a multi-channel valve, which is linked with a SCX column and TiO2 (titanium dioxide) column, an automated selection of three different experiment modes is achieved. Because the system uses the same solvent flow paths, the same SPE (solid phase extraction) column, and the same separation column for reverse-phase separation in 1D, 2D, and phosphopeptides enrichment experiments, the reproducibility of elution time obtained from the these experiments is in great agreement. Furthermore, fast online digestion was attempted using pepsin in a modified high pressure LC system. The system was possible to generate the high pressure condition (~10,000 psi) to accelerate enzyme digestion and reduced sample digestion time from hours to minutes. This first attempted development of the automated multidimensional and multifunctional ultra-high-pressure LC system is proven to be substantially reproducible, convenient and efficient compared with existing LC system. It also demonstrates potential to effectively evolve into a fully automated UPLC system suitable for high throughput proteomics analysis.