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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Specific Proteins Extraction by Antibody Conjugated Nanoparticles and Its Application to Biomarker Validation

2012년 3월 1일 22시 29분 49초
ANAL.P-646 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
백승훈, 이광렬, 이상원
고려대학교 화학과, Korea
The detection of diseases in their early stages is important to the success of therapeutic treatment. And it has been assisted by the discovery of altered tissue and fluid protein markers. However, current technologies have limited the analysis of the human proteome; especially, discovery of tumor-derived biomarkers directly is challenging because the abundant proteins impede detection of lower abundance tumor antigens. Here, to overcome that obstacle, specific extraction of target protein was described by using magnetic nanoparticles (NPs). NPs have been developed for an increasing number of applications in biological analysis. Compared with conventional micron-sized bead techniques, the NPs give the advantages such as higher surface-to-volume ratio, higher specificity, higher miscibility and faster binding time. In this study, for advanced target-specific interaction with C-reactive protein (CRP), CRP antibody loaded NPs were used. First, N-Succinimidyl 3-(2-pyridyldithio) propionate (SPDP) functionalized superparamagnetic Fe3O4@SiO2 NPs (ca. 27 nm diameter) were synthesized. Fe3O4@SiO2 NPs were coated 3-(Trihydroxysilyl) propylmethylphosphonate (THPMP) and 3-Aminopropyltriethoxysilane (APTS). Then, SPDP was modified on the surface of the Fe3O4@SiO2 NPs coated with THPMP and APTS. Cysteine-tagged protein G was loaded SPDP NPs through disulfide bonding. Using the specific interaction with CRP antibody, protein G-SPDP NPs were modified to specific enrichment of CRP from complex samples. Also, protein G-Dynabeads were used to compare the efficiency between NPs and micron-sized beads. The specific extraction of CRP was confirmed by both SDS-PAGE and LC-MS/MS experiments.