Inquiry on Abstract abstract@kcsnet.or.kr

Inquiry on Payment member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 12일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

110th General Meeting of Korean Chemical Society Buforin IIb as a peptide vector for the nonviral delivery of nucleic acids

Submission Date :
9 / 9 / 2012 , 11 : 50 : 02
Abstract Number :
Presenting Type:
Poster Presentation
Presenting Area :
Authors :
김지수, 하상수
경희대학교 화학과, Korea
Assigned Code :
BIO.P-764 Assigend Code Guideline
Presenting Time :
10월 17일 (수요일) 16:00~19:00
RNA interference (RNAi) is a natural response of eukaryotic cells to double-stranded RNA (dsRNA) leading to silencing of homologous gene transcripts by ~21 nucleotide (nt) dsRNAs, termed short interfering RNAs (siRNAs). While siRNAs have been rapidly appreciated to silence genes, efficient and non-toxic vectors for primary cells and for systemic in vivo delivery remain lacking. Several siRNA-delivery vehicles, including cell-penetrating peptides (CPPs), have been developed but their utility is often restricted by entrapment following endocytosis. Hence, developing CPPs that promote endosomal escape is a prerequisite for successful siRNA implementation. We here present buforin IIb, a synthetic analog of buforin II that contains a proline hinge between the two α-helices and a model α-helical sequence at the C-terminus (3× RLLR), in order to facilitate endosomal release of siRNAs. Stable buforin IIb-conjugated siRNAs enter entire cell populations and rapidly promote endosomal escape, resulting in robust RNAi responses in MCF-7 cells, with buforin IIb-mediated delivery being independent of cell confluence. These results imply that the peptide, in addition to having utility for RNAi screens in vitro, displays great therapeutic potential.