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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Identification of Antioxidative and Cholinesterase Inhibitory Compounds from Sargassum ringgoldianum

2014년 8월 28일 14시 07분 17초
MEDI.P-958 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
이제성, 이혜숙, 신가영, 이봉호1,*, 최병욱2,*
한밭대학교 생명공학과, Korea
1한밭대학교 응용화학생명공학부, Korea
2한밭대학교 화학생명공학과, Korea
In our continuing search for antioxidative and anti-cholinesterase compounds from marine alga, Sargassum ringgoldianum was examined and here we report the progress on the purification and structural determination of anti-cholinesterase compounds. From the 100 % methanol extract of S. ringgoldianum (300g), solvent partition fractions were prepared by using n-hexane(SRH), chloroform(SRC), butanol(SRB) and water(SRW). (SRH : 2.72g, SRC : 1.93g, SRB : 1.63g, SRW : 20.5g) Among them chloroform fraction (SRC) showed strong antioxidative and BuChE* inhibitory activities. The SRC of S. ringgoldianum showed about 56.0 % DPPH radical scavenging activity and 86.0 % BuChE inhibitory activity at 0.1mg/mL. Using various chromatographic methods and by the bioassay-guided fractionation, the SRC fraction was separated into six fractions, and each fraction showed antioxidative activity as 71.4, 88.8, 79.0, 79.7, 40.4, 34.1% at 0.1mg/mL, respectively. Especially, SRC-1 ~ SRC-4 showed strong antioxidative activities. The SRC-4 fraction (145mg) was further fractionated into 10 fractions(SRC-4-1~SRC-4-10) using preparative HPLC (Column : COSMOSIL packed 5C18, 20x250mm, 5um, Eluent : 90% MeOH, Flow rate : 5.0ml/min, UV : 220nm) and the SRC-4-6 (24.0mg) was further purified to give a single compound A (7.22mg). The structural analysis and biological activities of compound A will be reported. * BuChE : butyrylcholinesterase