A water-soluble boronate-based fluorescent probe was used to detect the peroxynitrite(ONOO-) in the monosaccharides(eg. D-fructose). When the boronate probe interacts with D-fructose, the fluoroscence intensity increases, but this complex in the presence of ONOO- has very low intensity. While, ROS/RNS also led to decrease the fluoroscence intensity but decrease less than ONOO- because of protection by the internal N-B iteraction. The interaction of probe with D-fructose can protect the boronic acid to oxidation by ROS/RNS. So, the boronate-based sugar complex can reacts preferentially with ONOO-. ONOO- largely influences the cell signaling and apoptosis under physiological conditions.
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