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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Origin of oscillatory feature in bacterial transcription rate fluctuation

2014년 8월 28일 16시 47분 59초
PHYS.P-460 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
박성준, 임유림, 양길석, 송상근, 김지현*, 성재영*
중앙대학교 화학과, Korea
Recently, we revealed that transcription rate autocorrelation function can show an oscillatory behavior over time by quantitatively analyzing the steady-state statistics of mRNA copy number per cell in Escherichia coli. In terms of time-dependent standard deviation of mRNA copy number, which is closely related to the transcription rate autocorrelation, we found from stochastic simulation that such an oscillatory behavior can be produced when the respective distributions of times required to complete successful initiation and elongation have a highly sub-Poissonian character and the mean time of RNA polymerase (RNAP) binding to promoter is sufficiently small compared with the mean initiation time, leading to a well-separated sequential alignment of RNAPs on a single gene. In addition to the experimental evidences showing that the successful initiation time distribution can be narrow enough to generate such an oscillatory behavior in the standard deviation, we investigate if the transcriptional elongation time distribution is also really the case by using the stochastic simulation algorithm constructed with the results of single-RNAP tracking experiments. Transcriptional pausing, which is contrasted to active elongation phase, is a key ingredient to realize heterogeneity in transcriptional elongation time in silico. It is shown that the resulting transcriptional elongation time distribution is highly sub-Poissonian.