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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Classification of bacteria using ESI lipid spectra

2014년 9월 4일 16시 29분 07초
BIO.P-659 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
류주연, 문정희1,*, 박병철2
한국생명공학연구원(KRIBB) 기능유전체연구센터, Korea
1한국생명공학연구원 단백체의학연구센터, Korea
2한국생명공학연구원(KRIBB) 유전체연구소, Korea
Identification of bacteria has been performed with GC-MS by obtaining fatty acid methyl ester (FAME) spectra and statistical treatment. Though FAME can be used successfully for identifying bacteria, recent development of ionization method leads us to use higher mass molecule since recording large molecular weight became easy. So, there has been attempt to identify bacteria using lipid profile using electrospray ionization (ESI), low temperature plasma (LTP), and so on. ESI is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. It is especially useful in producing ions from macromolecules because it overcomes the propensity of these molecules to fragment when ionized. The ESI spray requires little or no sample preparation prior to analysis and thus allows the sample to be examined rapidly in a near native state. In this study, four bacteria are cultivated 12 hr and 24 hr in LB agar plate. The bacteria are harvested with 1.5ml PBS. PBS was removed after mild centrifugation. Harvested samples were resuspended with 100% MeOH and directly analyzed by ESI-MS. The positive and the negative ion mode mass spectra were recorded for each bacterial sample with a scan range of 100?1400 m?z. The spectra obtained with both methods are compared with each other including the different culture duration. The plates are cultivated for each bacterium and their spectral patterns are analyzed by principal component analysis (PCA). The feasibility of identifying bacteria with lipid spectra and its field application is discussed.