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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Characterization of egg yolk plasma using asymmetrical flow field-flow fractionation coupled with multiple detectors

2014년 9월 4일 16시 42분 50초
ANAL.P-597 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
DouHaiyang, 최재영, 유영석, Emma Magnusson1, Lars Nilsson1, Björn Bergenståhl1, 이승호*
한남대학교 화학과, Korea
1Lund university, Sweden
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승인 3건
In this study, the capability of asymmetrical flow field-flow fractionation (AF4) coupled online with a UV/VIS, quasi-elastic light scattering (QELS), multiangle light scattering (MALS) and fluorescence (FS) detectors (AF4-UV/VIS-QELS-MALS-FS) was evaluated for separation and characterization of egg yolk plasma. AF4 provided gentle separation of the major components of the egg yolk plasma such as free proteins, low density lipoproteins (LDL) and their aggregates, according to their hydrodynamic sizes. Free proteins were completely separated from LDL by a programmed AF4 analysis, where the cross-flow was gradually reduced during a run. For each component, QELS and MALS yielded hydrodynamic radius (Rh) and radius of gyration (Rg) , respectively, allowing study on conformation (e.g., shape) of each component based on the ratio, Rg/Rh. The Rg/Rh data were confirmed by Cryo-transmission electron microscopy (Cryo-TEM) images of collected AF4 fractions. The Rg/Rh results indicated that LDL has spherical shape, while larger aggregates have swollen micro-gel structure. Elution of LDL was confirmed by staining with a fluorescent dye, Nile red. Collected fractions of free proteins were further characterized using a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results suggested that the absence of Ca2+ leads to aggregation, while the presence of Ca2+ leads to bindings of proteins. It was shown that AF4-UV/VIS-QELS-MALS-FS is a gentle and useful tool for analysis of egg yolk plasma.