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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Interferometric Scattering Microscopy for Investigating Self-Assembly Dynamics

2014년 9월 11일 10시 42분 02초
PHYS.P-493 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
김진현, 이호재*
광주과학기술원(GIST) 화학과, Korea
Self-assembly is a type of spontaneous association of components via non-covalent interactions (e.g. van der Waals interaction, hydrogen bonds, π-π interaction) where a disordered state becomes an organized one. For example, in an organism, self-assembly occurs at tissue formation where helical and chiral macromolecules such as collagen are secreted. The exact dynamics of self-assembly processes are still unclear, although they are very common and fundamental in nature. The goal of this experiment is to directly visualize the processes of self-assembly and to comprehend their dynamics better.
For this goal we choose the M13 phage as a model system mainly because of its dimension (800nm-length and 10-nm diameter) that allows optical observation. Since the M13 phage is transparent, we use a scattering microscope to observe it, and the scattered light is detected in the form of interference with the reflected light on a substrate. Thus, the name of the apparatus is interferometric scattering (iSCAT) microscope. Furthermore, we focus the narrow interfacial region between M13 phage solution and a solid substrate where the M13 phage self-assembles as its concentration increases by solvent evaporation. One of the possible setups is that M13 phage forms a film by pulling substrates vertically from phage solution at exactly constant controlled speeds.