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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Novel Electrochemical Biosensor Based on Quantum Dot Dissolution for a PMI Marker Detection

2016년 9월 1일 11시 40분 52초
ELEC.P-592 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 13일 (목요일) 11:00~12:30
저자 및
정봉진, Akter Rashida, Md. Aminur Rahman*
충남대학교 분석과학기술대학원, Korea
GAPDH (GD) is a protein which can be found frequently at the human body such as saliva and kidney and it can be utilized in forensic science such as postmortem interval (PMI) detection. GD is very important as its concentration decreases with time after death. This characteristic of GD protein can be utilized as a biomarker for developing a PMI biosensor system in saliva. The PMI biosensor was fabricated by immobilizing a monoclonal anti-GAPDH antibody on the functionalized cadmium selenide (CdSe) quantum dots (QD), which were attached on the self-assembled monolayer (SAM) of cysteamine containing graphene oxide (GO). The GAPDH detection was made through the dissolution of the surface attached CdSe QD by hydrogen peroxide, which was generated through the glucose oxidase (GOx) catalyzed β-glucose oxidation. GOx was used as an enzymatic label that was conjugated to GD protein through glutaraldehyde cross-linking. For enhancing the sensitivity by reducing the sensing layer, we performed the competitive assay in which GD-GOx conjugates and free-GD competed for binding to the active sites of antibody. The current response resulted from the CdSe dissolution was found to be decreased with increasing concentration of free GD and was proportional to the free-GD concentration. Thus, it was possible to quantify the free GD with this strategy and differential pulse voltammetry (DPV) technique was used to determine the analytical characteristics such as detection limit (DL) and linear dynamic range of GD detection, selectivity, stability, and the real sample analysis for the GD detection.