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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Effect of light on size of chlorella sorokiniana and production of glutathione using gravitational field-flow fractionation (GrFFF)

2017년 8월 24일 16시 05분 00초
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금 09시 : 52분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Yeowoon Koo, Jaeyeong Choi1, Seungho LEE1,*
Department of chemistry, Hannam University, Korea
1Department of Chemistry, Hannam University, Korea
chlorella sorokiniana(or simply, chlorella) is one of single-cell green microalgae and has the size range of 2~10 μm. It plays an important role in food as it provides a rich source of carbohydrates, vitamins and proteins. It can also produce the glutathione, which is one of important antioxidant in plants, animals, fungi and some bacteria. The antioxidant-production capacity of the chlorella sorokiniana is determined by various parameters including pH, temperature, and the presence of light. Previous studies have been mainly focused on the application of the chlorella, and basic studies on the relationship between the antioxidant-production capacity of the chlorella sorokiniana and these parameters are insufficient. In this work, the change in the size of the chlorella sorokiniana with the presence of light was studied, and then the effects of the size, the presence of light, and incubation time on the capacity of the chlorella sorokiniana a for production of glutathione were investigated. The size of chlorella sorokiniana was determined using an optical microscopy (OM) and gravitational field-flow fractionation (GrFFF). GrFFF is a gentle elution-based separation technique that is known to be useful for separation of micron-sized particles. The glutathione produced from chlorella was analyzed quantitatively using a UV/Vis spectrophotometry. Both the size and number of chlorella sorokiniana were increased with increasing incubation time until 96 hours, and then were remained constant. The concentration of glutathione also was increased with the incubation time until 96 hours, after which was decreased.