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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-580 |
| Subject |
Development of a facile biosensor for detecting norovirus using new specific binding peptides |
| Authors |
KIM MINWOO, Seung Hoon Back, Tae Jung Park*
Department of Chemistry, Chung-Ang University, Korea
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| Abstract |
Norovirus is one of the infectious disease in the world by eating raw foods which were infected by others, and there are many people could be infected to touch to be contaminated things by norovirus. It is a major cause of foodborne and nosocomial outbreaks. In this study, thoroughly and highly sensitive biosensors for detecting norovirus by applying a recognized affinity peptide as a platform were described. As used in this experiment electrochemical method is a cost-effective, fast responsive and easy to integrate information into miniaturized micro-devices like a portable biosensor. The performance of the peptides has been studied with fluorescent optical assay, and gold-immobilized synthetic peptides has been studying cyclic voltammetry(CV) and impedance spectroscopy (EIS). We found that several kinds of peptides (Noro-BP, nonFoul, (FelxL)₂, nonFoul(FlexL)₂) are the efficient recognizes for norovirus screened by using the phage display method. The performance of the above four peptides is evaluated by comparing real norovirus samples with rotavirus as a negative control. These peptides were effectively applied to electrochemistry method to detect the real norovirus sample. Among them, nonFoul(FlexL)₂ shows the best performance as the lowest detection limit value of 1.7 copies/mL compared with other peptides with real norovirus samples. In addition, the result could be expected that using nanoflowers’ with large surface area will be useful to peptide-based detection sensor for norovirus. These results suggest that the biosensor consists of peptide, has affinity to norovirus as a molecular binder and will be used to micro-device as a diagnostic tool. Moreover, the biosensor could be helped as a new biosensing platform for point-of-care testing by applying much more fields. |
| E-mail |
laugh_s@naver.com |
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121st General Meeting of the KCS